Abstract: To improve flue-cured tobacco seedlings with stronger root system, we compared seedling production on moist tray with seedling nursing on float system and seedling production on tray to select the best method of seedling production in central Henan Province. The results showed that seeds germinated and emergenced up to one week earlier in moist tray treatment than the other two treatments. Compared with the other two treatments, the whole seedling growth period with moist tray was shortened by 7-11 days. Furthermore, the seedlings were sound, and the plant height, stalk circumference and leaves were well developed, the stress resistance of tobacco plant was enhanced. The root system was well developed. The lateral root per plant increased by 72.6 strips and the dry mass of root system increased by 0.034 g. The seedling production on moist tray could be extended as a new seedling production method in tobacco production areas.